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KMID : 0368420200630040279
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Journal of Plant Biology
2020 Volume.63 No. 4 p.279 ~ p.288
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The Chitin-Induced Chimeric LYK4-ER Gene Improves the Heat Tolerance of Arabidopsis at the Seedling Stage
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Chen Linxiao
Xia Wei
Song Jinxing
Wu Mengqi
Xu Zhizhen
Hu Xiangyang
Zhang Wenqing
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Abstract
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Due to global warming, high temperature has become the main abiotic stress affecting plant growth worldwide. LysM-containing receptor-like kinase 4 (LYK4) is the receptor for chitin, and ERECTA(ER) is a key factor in plant tolerance to high temperature. In this study, we constructed a chitin-induced chimeric LYK4-ER gene, in which the extracellular region and transmembrane domain of the LYK4 gene are fused with the intracellular region of the ER gene. Colony PCR, RT-PCR and western blot analyses of LYK4-ER transcription in plants, confirmed that the LYK4-ER gene was successfully constructed and transferred into Arabidopsis. The LYK4-ER gene localized to the cytomembrane and cytoplasm in vivo because of the binding properties of the transmembrane domain of the LYK4-ER gene to the cell membrane. The transgenic plants showed a higher germination rate and germination index as well as a shorter mean germination time than the wild-type plants, indicating that the LYK4-ER gene increases the heat tolerance of Arabidopsis. The lower H2O2 content and relative electrolytic leakage of the transgenic plants showed that the status of these plants under heat stress was improved. UPLC-MS/MS was used to analyze the phytohormones content, which suggested that the transgenic plants exhibited improved heat tolerance through jasmonic acid signal transduction pathways.
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KEYWORD
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Chitosan, Chimeric gene, Heat stress, Germination, Phytohormones
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